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oppB [2019-06-27 14:15:36]
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oppB [2019-06-27 14:15:36]

oligopeptide ABC transporter (permease)
Locus
BSU11440
Isoelectric point
9.86
Molecular weight
33.92 kDa
Protein length
311 aa Sequence Blast
Gene length
936 bp Sequence Blast
Function
initiation of sporulation, competence development
Product
oligopeptide ABC transporter (permease)
Essential
no
Synonyms
spo0KB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
  • Information processing → Genetics → Genetic competence
  • Information processing → Regulation of gene expression → phosphorelay
  • Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
  • Lifestyles → Sporulation → phosphorelay
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,221,594 → 1,222,529

    The protein

    Catalyzed reaction/ biological activity

  • required for the uptake of quorum sensing peptides such as PhrH PubMed

    • Protein family

  • Binding-protein-dependent transport system permease family (according to UniProt)

    • Paralogous protein(s)

  • AppB, DppB

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    oppA-oppB-oppC-oppD-oppF
    Description
    PubMed

    Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, no derepression occurs, however, in a CodY mutant, due to increased repression by ScoC PubMed, in CodY regulon
  • ScoC: repression, PubMed, in ScoC regulon

    • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

    • Additional information

  • A ncRNA is predicted between 'trpS' and 'oppA' PubMed
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    Biological materials

    Mutant

  • GP2063 (oppB::spc), available in Jörg Stülke's lab
  • GP2097 (D(oppA-oppB-oppC-oppD-oppF)::aphA3), available in Jörg Stülke's lab
  • BKE11440 (ΔoppB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGGGGAACACCTCCATG, downstream forward: _UP4_AAAGCAAAGAAAGGAGCCTA
  • BKK11440 (ΔoppB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGGGGAACACCTCCATG, downstream forward: _UP4_AAAGCAAAGAAAGGAGCCTA

    • References

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